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Imagerie en temps réel des jonctions endothéliales cellule-cellule Pendant neutrophiles Transmigration Sous physiologique débit
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow
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Imagerie en temps réel des jonctions endothéliales cellule-cellule Pendant neutrophiles Transmigration Sous physiologique débit

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19,239 Views

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11:26 min

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August 14, 2014

DOI:

10.3791/51766-v

DOI:
10.3791/51766-v

•

11:26 min
August 14, 2014

•

1 Views
, , ,
1Department of Molecular Cell Biology,Sanquin Research and Landsteiner Laboratory, AMC at University of Amsterdam

Summary

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Les leucocytes traversent la monocouche endothéliale utilisant le paracellulaire ou la voie transcellulaire. Nous avons développé un test simple de suivre la distribution de jonction endogène VE-cadhérine et PECAM-1 au cours de la migration leucocytaire transendotheliale sous flux physiologique à une discrimination entre les deux voies de transmigration.

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Cite this Article

Kroon, J., Daniel, A. E., Hoogenboezem, M., van Buul, J. D. Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow. J. Vis. Exp. (90), e51766, doi:10.3791/51766 (2014).

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