Journal
/
/
神经元的密集核心囊泡的超分辨率成像
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Super-resolution Imaging of Neuronal Dense-core Vesicles
DOI:

09:30 min

July 02, 2014

, , ,

Chapters

  • 00:05Title
  • 01:12Image Acquisition
  • 05:36Image Processing, Display, and Analysis
  • 07:07Results: Representative PALM Images
  • 09:04Conclusion

Summary

Automatic Translation

我们描述了如何实现光敏定位显微镜(PALM)为基础的固定,培养的神经元囊泡的研究。我们的协议的关键组件包括标签囊泡photoconvertible嵌合体,采集稀疏采样的原始图像的超分辨率显微镜系统,以及处理原始图像来产生超分辨率图像。

Related Videos

Read Article