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Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
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Neuroscience
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JoVE Journal Neuroscience
Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

DOI:
10.3791/56776-v

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10:35 min

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March 15, 2018

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,
1CHU de Québec-Université Laval Research Center,Université Laval, 2Department of Biochemistry, Microbiology and Bioinformatics,Université Laval

Chapters

  • 00:04Title
  • 00:46Hippocampal Slice Preparation
  • 02:03Whole-cell Patch-clamp Recordings
  • 04:33Two-photon Ca2+ Imaging
  • 08:36Results: Postsynaptic Ca2+ Transients Evoked by a Burst of Electrical Stimulation
  • 09:49Conclusion

Summary

Automatic Translation

Automatic Translation

We present a method combining whole-cell patch-clamp recordings and two-photon imaging to record Ca2+ transients in neuronal dendrites in acute brain slices.

Tags

Two-photon Calcium ImagingNeuronal DendritesBrain SlicesWhole-cell Patch-clampCalcium SignalingSynaptic InputsSpatiotemporal ResolutionACSF-sucroseVibratomeHippocampal SlicesACSF-recoveryTwo-photon MicroscopeInfrared Differential Interference Contrast MicroscopyStimulating PipettePatch PipetteMultiClamp AmplifierVoltage Clamp

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