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Super-resolution Imaging of Neuronal Dense-core Vesicles
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JoVE Journal Neurowissenschaften
Super-resolution Imaging of Neuronal Dense-core Vesicles

Super-resolution Imaging of Neuronal Dense-core Vesicles

9,607 Views

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09:30 min

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July 02, 2014

DOI:

10.3791/51394-v

DOI:
10.3791/51394-v

•

09:30 min
July 02, 2014

•

3 Views
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1Department of Physics,Lewis & Clark College, 2Program in Biochemistry and Molecular Biology,Lewis & Clark College, 3Jungers Center for Neuroscience Research,Oregon Health & Science University, 4Department of Chemistry,Lewis & Clark College

Summary

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We describe how to implement photoactivated localization microscopy (PALM)-based studies of vesicles in fixed, cultured neurons. Key components of our protocol include labeling vesicles with photoconvertible chimeras, collecting sparsely sampled raw images with a super-resolution microscopy system, and processing the raw images to produce a super-resolution image.

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Scalettar, B. A., Shaver, D., Kaech, S., Lochner, J. E. Super-resolution Imaging of Neuronal Dense-core Vesicles. J. Vis. Exp. (89), e51394, doi:10.3791/51394 (2014).

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