ROS Live Cell Imaging During Neuronal Development

Aslihan Terzi; S. M. Sabbir Alam; Daniel M. Suter

doi:10.3791/62165

Journal

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Neuroscience

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ROS Live Cell Imaging During Neuronal Development

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

ROS Live Cell Imaging During Neuronal Development

3,970 Views

•

09:25 min

•

February 09, 2021

DOI:

10.3791/62165-v

DOI:

10.3791/62165-v

•

09:25 min

February 09, 2021

•

4 Views

Aslihan Terzi^1,2, S. M. Sabbir Alam^1,2, Daniel M. Suter^1,2,3

¹Department of Biological Sciences,Purdue University, ²Purdue Institute for Integrative Neuroscience,Purdue University, ³Bindley Bioscience Center,Purdue University

Summary

This protocol describes the use of a genetically encoded hydrogen peroxide (H₂O₂)-biosensor in cultured zebrafish neurons and larvae for assessing the physiological signaling roles of H₂O₂ during nervous system development. It can be applied to different cell types and modified with experimental treatments to study reactive oxygen species (ROS) in general development.

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ROS Live Cell Imaging During Neuronal Development

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