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In vivo Calcium Imaging in Mouse Inferior Olive
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
In vivo Calcium Imaging in Mouse Inferior Olive

In vivo Calcium Imaging in Mouse Inferior Olive

DOI:
10.3791/62222-v

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08:58 min

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June 10, 2021

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, ,
1Neuronal Rhythms in Movement unit,OIST

Chapters

  • 00:00Introduction
  • 00:57Protocol
  • 01:45Administration of Anesthesia and Preparation of the Mouse for the Surgery
  • 02:42Tracheotomy and Intubation
  • 05:02Exposing the Brainstem
  • 06:42Calcium Imagin
  • 07:55Representative Results
  • 08:20Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to expose the brainstem of adult mouse from the ventral side. By using a gradient-refractive index lens with a miniature microscope, calcium imaging can be used to examine the activity of inferior olive neural somata in vivo.

Tags

In VivoCalcium ImagingMouseInferior OliveBrain StemGRIN LensesNeuronal ActivityCalcium SensorsSpatial-temporal Activity PatternsInput IntegrationSurgical SkillsTracheotomyKetamineIsofluraneAnesthesiaBlunt DissectionTracheaSternothyroid Muscle

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