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Isolierung und chemische Charakterisierung von Lipid A von gramnegativen Bakterien
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Chemistry
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JoVE Journal Chemistry
Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
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Isolierung und chemische Charakterisierung von Lipid A von gramnegativen Bakterien

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31,135 Views

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12:57 min

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September 16, 2013

DOI:

10.3791/50623-v

DOI:
10.3791/50623-v

•

12:57 min
September 16, 2013

•

11 Views
, , ,
1Section of Molecular Genetics and Microbiology,The University of Texas at Austin, 2Department of Chemistry and Biochemistry,The University of Texas at Austin, 3The Institute of Cellular and Molecular Biology,The University of Texas at Austin

Summary

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Isolierung und Charakterisierung der Lipid-A-Domäne von Lipopolysaccharid (LPS) von gramnegativen Bakterien gibt Einblick in Zelloberfläche basierend Mechanismen der Antibiotikaresistenz, bakterielle Überlebensrate und Fitness, und wie chemisch unterschiedlicher Lipid einer molekularen Spezies unterschiedlich modulieren Host angeborenen Immunantwort.

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Cite this Article

Henderson, J. C., O'Brien, J. P., Brodbelt, J. S., Trent, M. S. Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria. J. Vis. Exp. (79), e50623, doi:10.3791/50623 (2013).

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