Journal
/
Chemie
/
Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
JoVE Journal
Chemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Chemie
Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria

31,139 Views

•

12:57 min

•

September 16, 2013

DOI:

10.3791/50623-v

DOI:
10.3791/50623-v

•

12:57 min
September 16, 2013

•

11 Views
, , ,
1Section of Molecular Genetics and Microbiology,The University of Texas at Austin, 2Department of Chemistry and Biochemistry,The University of Texas at Austin, 3The Institute of Cellular and Molecular Biology,The University of Texas at Austin

Summary

Automatically generated

Isolation and characterization of the lipid A domain of lipopolysaccharide (LPS) from gram-negative bacteria provides insight into cell surface based mechanisms of antibiotic resistance, bacterial survival and fitness, and how chemically diverse lipid A molecular species differentially modulate host innate immune responses.

Explore More Videos

LipopolysaccharideLipid AGram-negative BacteriaCell Surface MoleculeChemical CharacterizationMass SpectrometryThin Layer ChromatographyInnate Immune ResponseAntibiotic Resistance

Read Article

Cite this Article

Henderson, J. C., O'Brien, J. P., Brodbelt, J. S., Trent, M. S. Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria. J. Vis. Exp. (79), e50623, doi:10.3791/50623 (2013).

Download .ris file

Copy

Share Video

.

Copy