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Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
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Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria

31,139 Views

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12:57 min

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September 16, 2013

DOI:

10.3791/50623-v

DOI:
10.3791/50623-v

•

12:57 min
September 16, 2013

•

11 Views
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1Section of Molecular Genetics and Microbiology,The University of Texas at Austin, 2Department of Chemistry and Biochemistry,The University of Texas at Austin, 3The Institute of Cellular and Molecular Biology,The University of Texas at Austin

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Isolation and characterization of the lipid A domain of lipopolysaccharide (LPS) from gram-negative bacteria provides insight into cell surface based mechanisms of antibiotic resistance, bacterial survival and fitness, and how chemically diverse lipid A molecular species differentially modulate host innate immune responses.

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LipopolysaccharideLipid AGram-negative BacteriaCell Surface MoleculeChemical CharacterizationMass SpectrometryThin Layer ChromatographyInnate Immune ResponseAntibiotic Resistance

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Henderson, J. C., O'Brien, J. P., Brodbelt, J. S., Trent, M. S. Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria. J. Vis. Exp. (79), e50623, doi:10.3791/50623 (2013).

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