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从人类多能干细胞的规范在前脑谷氨酸能神经元

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

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从人类多能干细胞的规范在前脑谷氨酸能神经元

DOI:

10.3791/50321-v

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10:49 min

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April 14, 2013

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Erin M. Boisvert², Kyle Denton, Ling Lei, Xue-Jun Li³

¹Department of Neuroscience,The University of Connecticut Health Center, ²Department of Genetics and Developmental Biology,The University of Connecticut Health Center, ³Stem Cell Institute,The University of Connecticut Health Center

Chapters

00:05Title
01:39Generation of Human Pluripotent Stem Cell Aggregates
03:53Induction of Neuroepithelial Cells
05:11Generation of Telencephalic Progenitors
06:33Differentiation into Telencephalic Neurons
08:18Telencephalic Neuronal Generation
10:11Conclusion

Summary

Automatic Translation

此过程产生在前脑的神经元通过检查站，这是人类发展过程中所观察到的类似。细胞可自发地分化，暴露因素推动对神经系，是孤立的，并镀上盖玻片，以便终末分化和成熟。

Tags

Telencephalic Glutamatergic Neurons Human Pluripotent Stem Cells Differentiation Process Clumps Aggregates Suspension Culture HESC Medium Spontaneous Differentiation Three Germ Layers Neural Induction Medium Neuroepithelial Cells Neurospheres Coverslips Telencephalic Precursors Dorsal Telencephalic Progenitors Glutamatergic Neurons Development Of Neurons Diseases Affecting Neurons

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