Journal
/
神经科学
/
The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

10,793 Views

•

10:49 min

•

April 14, 2013

DOI:

10.3791/50321-v

DOI:
10.3791/50321-v

•

10:49 min
April 14, 2013

•

4 Views
, , ,
1Department of Neuroscience,The University of Connecticut Health Center, 2Department of Genetics and Developmental Biology,The University of Connecticut Health Center, 3Stem Cell Institute,The University of Connecticut Health Center

Summary

Automatically generated

This procedure yields telencephalic neurons by going through checkpoints which are similar to those observed during human development. The cells are allowed to spontaneously differentiate, are exposed to factors which push them towards the neural lineage, are isolated, and are plated onto coverslips to allow for terminal differentiation and maturation.

Explore More Videos

Human Pluripotent Stem CellsTelencephalic Glutamatergic NeuronsNeural InductionNeuroepithelial CellsTelencephalic PrecursorsDorsal Telencephalic ProgenitorsGlutamatergic Neurons

Read Article

Cite this Article

Boisvert, E. M., Denton, K., Lei, L., Li, X. The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells. J. Vis. Exp. (74), e50321, doi:10.3791/50321 (2013).

Download .ris file

Copy

Share Video

.

Copy