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The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells
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The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

10,793 Views

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10:49 min

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April 14, 2013

DOI:

10.3791/50321-v

DOI:
10.3791/50321-v

•

10:49 min
April 14, 2013

•

4 Views
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1Department of Neuroscience,The University of Connecticut Health Center, 2Department of Genetics and Developmental Biology,The University of Connecticut Health Center, 3Stem Cell Institute,The University of Connecticut Health Center

Summary

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This procedure yields telencephalic neurons by going through checkpoints which are similar to those observed during human development. The cells are allowed to spontaneously differentiate, are exposed to factors which push them towards the neural lineage, are isolated, and are plated onto coverslips to allow for terminal differentiation and maturation.

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Cite this Article

Boisvert, E. M., Denton, K., Lei, L., Li, X. The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells. J. Vis. Exp. (74), e50321, doi:10.3791/50321 (2013).

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