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幼虫，蛹和成人的解剖和免疫组化<em>果蝇</em>视网膜
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Neuroscience
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JoVE Journal Neuroscience
Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas
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幼虫，蛹和成人的解剖和免疫组化<em>果蝇</em>视网膜

DOI:
10.3791/4347-v

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11:58 min

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November 14, 2012

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, , , , ,
1Department of Biology,New York University

Chapters

  • 00:05Title
  • 01:26Dissection of Larval Eye Imaginal Discs
  • 04:33Dissection of 50% Pupation Retinas
  • 07:42Dissection of Adult Eyes
  • 10:19Antibody Stained Retinas from Three Developmental Stages
  • 11:28Conclusion

Summary

Automatic Translation

Automatic Translation

“<em>果蝇</em>视网膜是一小部分的刻板的方式所产生的细胞类型组成的类似晶体的晶格<sup> 1</sup>。其顺从先进的遗传分析，使研究复杂的发展计划。本协议描述了视网膜的解剖和免疫组化在三个连续的发育阶段，与光感受器分化的重点。

Tags

DissectionImmunohistochemistryLarval Drosophila RetinasPupal Drosophila RetinasAdult Drosophila RetinasCompound EyeOmmatidiaCellsCone CellsPigment CellsBristle CellPhotoreceptors (PRs)RhabdomeresRhodopsins (Rhs)Rh1R1-R6 PRsR7 PRsR8 PRsSubtypesLarval Eye-antennal Imaginal DiscEpithelial CellsDifferentiation WaveOmmatidia AssemblyFounder Cell R8Pupal DevelopmentMorphological ChangesRhabdomere FormationSynaptogenesisRh Expression

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