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Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas
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November 14, 2012
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The Drosophila retina is a crystal-like lattice composed of a small number of cell types that are generated in a stereotyped manner 1. Its amenability to sophisticated genetic analysis allows the study of complex developmental programs. This protocol describes dissections and immunohistochemistry of retinas at three discrete developmental stages, with a focus on photoreceptor differentiation.
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DissectionImmunohistochemistryLarval Drosophila RetinasPupal Drosophila RetinasAdult Drosophila RetinasCompound EyeOmmatidiaCellsCone CellsPigment CellsBristle CellPhotoreceptors (PRs)RhabdomeresRhodopsins (Rhs)Rh1R1-R6 PRsR7 PRsR8 PRsSubtypesLarval Eye-antennal Imaginal DiscEpithelial CellsDifferentiation WaveOmmatidia AssemblyFounder Cell R8Pupal DevelopmentMorphological ChangesRhabdomere FormationSynaptogenesisRh Expression
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Hsiao, H., Johnston Jr., R. J., Jukam, D., Vasiliauskas, D., Desplan, C., Rister, J. Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas. J. Vis. Exp. (69), e4347, doi:10.3791/4347 (2012).
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