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Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas
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November 14, 2012
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Summary
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The Drosophila retina is a crystal-like lattice composed of a small number of cell types that are generated in a stereotyped manner 1. Its amenability to sophisticated genetic analysis allows the study of complex developmental programs. This protocol describes dissections and immunohistochemistry of retinas at three discrete developmental stages, with a focus on photoreceptor differentiation.
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DissectionImmunohistochemistryLarval Drosophila RetinasPupal Drosophila RetinasAdult Drosophila RetinasCompound EyeOmmatidiaCellsCone CellsPigment CellsBristle CellPhotoreceptors (PRs)RhabdomeresRhodopsins (Rhs)Rh1R1-R6 PRsR7 PRsR8 PRsSubtypesLarval Eye-antennal Imaginal DiscEpithelial CellsDifferentiation WaveOmmatidia AssemblyFounder Cell R8Pupal DevelopmentMorphological ChangesRhabdomere FormationSynaptogenesisRh Expression
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Cite this Article
Hsiao, H., Johnston Jr., R. J., Jukam, D., Vasiliauskas, D., Desplan, C., Rister, J. Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas. J. Vis. Exp. (69), e4347, doi:10.3791/4347 (2012).
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