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Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

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16,196 Views

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09:45 min

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June 03, 2011

DOI:

10.3791/2649-v

DOI:
10.3791/2649-v

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09:45 min
June 03, 2011

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3 Views
, , , , , , , ,
1Department of Zoology,University of Oklahoma – Norman, 2Department of Biology,Brandeis University

Summary

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We describe a Flippase-induced intersectional Gal80/Gal4 repression (FINGR) method, allowing tissue-specific FLP to determine Gal80 expression patterns. Wherever Gal4 and FLP overlap, Gal4 expression is turned on (Gal80 flipped out) or off (Gal80 flipped in). The FINGR method is versatile for clonal analysis and neural circuit mapping.

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Fore, T. R., Ojwang, A. A., Warner, M. L., Peng, X., Bohm, R. A., Welch, W. P., Goodnight, L. K., Bao, H., Zhang, B. Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS. J. Vis. Exp. (52), e2649, doi:10.3791/2649 (2011).

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