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Hipotalamik Fare Beyin Dilimleri GFP etiketli Nöronlar in Görüntüleme Kalsiyum Cevapları
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August 24, 2012
DOI:
Summary
Automatically generated
Bu protokol, biz görüntüleme Ca son gelişmeleri güncellemek<sup> 2 +</supBir kırmızı floresan Ca kullanarak beyin dokusunda dilimler GFP etiketli nöronlar> sinyalleri<sup> 2 +</sup> Gösterge boyası.
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ImagingCalcium ResponsesGFP-tagged NeuronsHypothalamic Mouse Brain SlicesEncoding Of InformationMolecular StepsNeurohormone Signal Transduction CascadesIntracellular Ca2+Synaptic SitesGreen Fluorescent Protein (GFP)Spatially And Temporally Stimulus-induced Ca2+ ResponsesNon-green Fluorescent Ca2+ Indicator DyeConfocal MicroscopyRatiometric Ca2+ Indicator Fura-2Ultraviolet (UV) Light
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Cite this Article
Schauer, C., Leinders-Zufall, T. Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices. J. Vis. Exp. (66), e4213, doi:10.3791/4213 (2012).
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