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Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
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09:14 min
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August 24, 2012
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概要
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In this protocol, we update recent progress in imaging Ca2+ signals of GFP-tagged neurons in brain tissue slices using a red fluorescent Ca2+ indicator dye.
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ImagingCalcium ResponsesGFP-tagged NeuronsHypothalamic Mouse Brain SlicesEncoding Of InformationMolecular StepsNeurohormone Signal Transduction CascadesIntracellular Ca2+Synaptic SitesGreen Fluorescent Protein (GFP)Spatially And Temporally Stimulus-induced Ca2+ ResponsesNon-green Fluorescent Ca2+ Indicator DyeConfocal MicroscopyRatiometric Ca2+ Indicator Fura-2Ultraviolet (UV) Light
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Schauer, C., Leinders-Zufall, T. Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices. J. Vis. Exp. (66), e4213, doi:10.3791/4213 (2012).
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