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Respostas de imagem de cálcio nos neurônios GFP-tagged fatias de cérebro de rato Hipotalâmicos
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09:14 min
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August 24, 2012
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Summary
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Neste protocolo, atualizar o progresso recente na imagem Ca<sup> 2 +</sup> Sinais de neurónios GFP-tagged em fatias de cérebro de tecido, utilizando um vermelho fluorescente Ca<sup> 2 +</sup> Corante indicador.
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ImagingCalcium ResponsesGFP-tagged NeuronsHypothalamic Mouse Brain SlicesEncoding Of InformationMolecular StepsNeurohormone Signal Transduction CascadesIntracellular Ca2+Synaptic SitesGreen Fluorescent Protein (GFP)Spatially And Temporally Stimulus-induced Ca2+ ResponsesNon-green Fluorescent Ca2+ Indicator DyeConfocal MicroscopyRatiometric Ca2+ Indicator Fura-2Ultraviolet (UV) Light
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Schauer, C., Leinders-Zufall, T. Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices. J. Vis. Exp. (66), e4213, doi:10.3791/4213 (2012).
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