Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices

Christian Schauer; Trese Leinders-Zufall

doi:10.3791/4213

Journal

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Neurociência

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Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices

17,681 Views

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09:14 min

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August 24, 2012

DOI:

10.3791/4213-v

DOI:

10.3791/4213-v

•

09:14 min

August 24, 2012

•

4 Views

Christian Schauer¹, Trese Leinders-Zufall¹

¹Department of Physiology, School of Medicine,University of Saarland, Homburg, Germany

Summary

In this protocol, we update recent progress in imaging Ca²⁺ signals of GFP-tagged neurons in brain tissue slices using a red fluorescent Ca²⁺ indicator dye.

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Imaging Calcium Responses GFP-tagged Neurons Hypothalamic Mouse Brain Slices Encoding Of Information Molecular Steps Neurohormone Signal Transduction Cascades Intracellular Ca2+Synaptic Sites Green Fluorescent Protein (GFP)Spatially And Temporally Stimulus-induced Ca2+ Responses Non-green Fluorescent Ca2+ Indicator Dye Confocal Microscopy Ratiometric Ca2+ Indicator Fura-2 Ultraviolet (UV) Light

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Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices

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