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Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
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Neurociência
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JoVE Journal Neurociência
Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices

Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices

DOI:
10.3791/4213-v

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09:14 min

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August 24, 2012

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1Department of Physiology, School of Medicine,University of Saarland, Homburg, Germany

Capítulos

  • 00:05Título
  • 01:52Solution Preparation and Brain Dissection
  • 03:21Coronal Hypothalamic Sections Slicing
  • 04:30Preparation of the Ca2+ Indicator Dye Loading Solution
  • 06:35Microscopy and Analysis
  • 07:59Results: Ca2+ Signals in TGFP Neurons of Mouse Hypothalamic Brain Slices
  • 08:52Conclusion

Summary

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In this protocol, we update recent progress in imaging Ca2+ signals of GFP-tagged neurons in brain tissue slices using a red fluorescent Ca2+ indicator dye.

Tags

ImagingCalcium ResponsesGFP-tagged NeuronsHypothalamic Mouse Brain SlicesEncoding Of InformationMolecular StepsNeurohormone Signal Transduction CascadesIntracellular Ca2+Synaptic SitesGreen Fluorescent Protein (GFP)Spatially And Temporally Stimulus-induced Ca2+ ResponsesNon-green Fluorescent Ca2+ Indicator DyeConfocal MicroscopyRatiometric Ca2+ Indicator Fura-2Ultraviolet (UV) Light

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