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Imaging Calcium Reacties in GFP-gelabelde neuronen van de hypothalamus Mouse Brain Slices
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August 24, 2012
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In dit protocol, updaten we de recente vooruitgang in de beeldvorming Ca<sup> 2 +</sup> Signalen van GFP-gemerkte neuronen in hersenweefsel plakjes met een rode fluorescerende Ca<sup> 2 +</sup> Indicator kleurstof.
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ImagingCalcium ResponsesGFP-tagged NeuronsHypothalamic Mouse Brain SlicesEncoding Of InformationMolecular StepsNeurohormone Signal Transduction CascadesIntracellular Ca2+Synaptic SitesGreen Fluorescent Protein (GFP)Spatially And Temporally Stimulus-induced Ca2+ ResponsesNon-green Fluorescent Ca2+ Indicator DyeConfocal MicroscopyRatiometric Ca2+ Indicator Fura-2Ultraviolet (UV) Light
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Schauer, C., Leinders-Zufall, T. Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices. J. Vis. Exp. (66), e4213, doi:10.3791/4213 (2012).
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