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Chapters
- 00:05Title
- 01:15Dissection and Dissociation of Intestinal Epithelial Cells
- 03:56Tissue Digestion and Isolation of Intestinal Cells
- 05:47Gating Strategy for Laimna Propria Antigen-presenting Cells
- 07:55Results: Cell Yield and Antibody Staining Quality Depends on Digestion Time
- 08:58Conclusion
Tags
IsolationCharacterizationDendritic CellsMacrophagesMouse IntestineInnate Immune CellsAdaptive Immune CellsToleranceCommensal FloraFood AntigensInflammatory ResponsesAntigen Presenting CellsDCsMacrophagesIntestinal Immune HomeostasisMicrobiotaIsolation MethodsDigestion TimesCell Surface Antigen ExpressionCell ViabilityCell YieldPhenotypic CharacterizationFluorescence-labeled Monoclonal AntibodiesFlow Cytometric AnalysisFunctional StudiesCD11cCD11b Magnetic-activated Cell Sorting Beads
