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Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
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Immunology and Infection
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JoVE Journal Immunology and Infection
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols

13,205 Views

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08:31 min

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October 18, 2016

DOI:

10.3791/54296-v

DOI:
10.3791/54296-v

•

08:31 min
October 18, 2016

•

5 Views
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1Department of Immunology, Herbert Wertheim College of Medicine,Florida International University, 2Millipore Sigma

Tags

MonocyteDendritic CellIsolationCharacterizationImaging Flow CytometryCell Surface ExpressionCD11cCD14PBMCAdherenceGMCSFIL4

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Figueroa, G., Parira, T., Laverde, A., Casteleiro, G., El-Mabhouh, A., Nair, M., Agudelo, M. Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols. J. Vis. Exp. (116), e54296, doi:10.3791/54296 (2016).

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