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Immunology and Infection
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Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

DOI:
10.3791/55445-v

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07:46 min

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April 03, 2017

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, , , , , , ,
1Department of Veterinary and Biomedical Sciences,The Pennsylvania State University, 2Microscopy and Cytometry Facility, The Huck Institutes of Life Sciences,Pennsylvania State University

Chapters

  • 00:05Title
  • 00:51Liver Tissue Isolation
  • 03:57Liver Cell Dissociation
  • 05:23Results: Representative Liver-isolated Macrophage Characterization
  • 06:59Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol allows a researcher to isolate and characterize tissue-resident macrophages in various hallmark inflamed tissues extracted from diet-induced models of metabolic disorders.

Tags

Macrophage IsolationPerfusionLiverObesity-related InflammationChronic InflammationImmunology

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