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Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

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09:25 min

May 21, 2012

DOI:

09:25 min
May 21, 2012

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Summary

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Here, we detail a methodology for the rapid isolation of mouse intestinal dendritic cells (DCs) and macrophages. Phenotypic characterization of intestinal DCs and macrophages is performed using multi-color flow cytometric analysis while magnetic bead enrichment followed by cell sorting is used to yield highly pure populations for functional studies.

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