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Um sistema de cultura primária Neuron para o Estudo da Herpes Simplex Virus latência e reativação
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1Department of Microbiology,New York University School of Medicine, 2Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine,New York University School of Medicine, 3Department of Otolaryngology,New York University School of Medicine, 4Department of Cell Biology,New York University School of Medicine, 5Department of Physiology and Neuroscience,New York University School of Medicine, 6Department of Psychiatry,New York University School of Medicine, 7Center for Neural Science,New York University School of Medicine
Chapters
- 00:05Title
- 01:52Isolation and Culture of SCG Neurons from Rat Embryos
- 05:56Infection of SCG Cultures with HSV-1 Us11-EGFP and Establishment of Latency
- 08:35Reactivation and Assessment
- 09:45Results: HSV-1 Reactivation
- 11:19Conclusion
O protocolo descreve um sistema de modelo eficiente e reprodutível para estudar vírus herpes simplex tipo 1 (HSV-1) a latência e reactivação. O ensaio emprega homogéneos culturas de neurónios simpáticos e permite a dissecção molecular do vírus de neurónios-interacções usando uma variedade de ferramentas, incluindo a interferência de RNA e de expressão de proteínas recombinantes.
