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A primaria del sistema cultura Neuron per lo Studio della latenza del virus Herpes Simplex e la riattivazione
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12:22 min
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April 02, 2012
DOI:
1Department of Microbiology,New York University School of Medicine, 2Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine,New York University School of Medicine, 3Department of Otolaryngology,New York University School of Medicine, 4Department of Cell Biology,New York University School of Medicine, 5Department of Physiology and Neuroscience,New York University School of Medicine, 6Department of Psychiatry,New York University School of Medicine, 7Center for Neural Science,New York University School of Medicine
Summary
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Il protocollo descrive un sistema modello efficiente e riproducibile per studiare herpes simplex virus di tipo 1 (HSV-1) latenza e riattivazione. Il dosaggio utilizza omogenei culture neurone simpatico e consente la dissezione molecolare del virus-neurone interazioni utilizzando una varietà di strumenti interferenza tra RNA e l'espressione di proteine ricombinanti.
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Kobayashi, M., Kim, J., Camarena, V., Roehm, P. C., Chao, M. V., Wilson, A. C., Mohr, I. A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation. J. Vis. Exp. (62), e3823, doi:10.3791/3823 (2012).
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