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12:22 min
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April 02, 2012
DOI:
1Department of Microbiology,New York University School of Medicine, 2Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine,New York University School of Medicine, 3Department of Otolaryngology,New York University School of Medicine, 4Department of Cell Biology,New York University School of Medicine, 5Department of Physiology and Neuroscience,New York University School of Medicine, 6Department of Psychiatry,New York University School of Medicine, 7Center for Neural Science,New York University School of Medicine
Summary
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프로토콜은 헤르페스 바이러스 1 형 (HSV-1) 대기 시간과 재활 성화를 단순 공부하는 효율적이고 재현성 모델 시스템을 설명합니다. 분석은 동질적인 교감 신경 세포 배양을 고용하고 RNA 간섭 및 재조합 단백질의 표현을 포함한 다양한 도구를 사용하여 바이러스 신경 세포 상호 작용의 분자 해부을 허용합니다.
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Kobayashi, M., Kim, J., Camarena, V., Roehm, P. C., Chao, M. V., Wilson, A. C., Mohr, I. A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation. J. Vis. Exp. (62), e3823, doi:10.3791/3823 (2012).
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