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Immunology and Infection

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JoVE Journal Immunology and Infection
A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation
 

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

Article DOI: 10.3791/3823-v • 12:22 min • April 2nd, 2012
April 2nd, 2012 •
1, 1, 2, 3, 2,4,5,6,7, 1, 1
1Department of Microbiology, New York University School of Medicine, 2Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine, New York University School of Medicine, 3Department of Otolaryngology, New York University School of Medicine, 4Department of Cell Biology, New York University School of Medicine, 5Department of Physiology and Neuroscience, New York University School of Medicine, 6Department of Psychiatry, New York University School of Medicine, 7Center for Neural Science, New York University School of Medicine

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The protocol describes an efficient and reproducible model system to study herpes simplex virus type 1 (HSV-1) latency and reactivation. The assay employs homogenous sympathetic neuron cultures and allows for the molecular dissection of virus-neuron interactions using a variety of tools including RNA interference and expression of recombinant proteins.

Tags

Primary Neuron Culture System Herpes Simplex Virus Latency Reactivation Antivirals Vaccines In Vivo Studies Small Animal Models Viral Gene Requirements Immune Responses Neuron-only System Tissue Culture Sympathetic Neurons Rat Superior Cervical Ganglia (SCG)
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