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Engineering and Evolution af syntetisk Adeno-associeret virus (AAV) Genterapivektorer via DNA Family blander
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Immunology and Infection
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JoVE Journal Immunology and Infection
Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling

Engineering and Evolution af syntetisk Adeno-associeret virus (AAV) Genterapivektorer via DNA Family blander

DOI:
10.3791/3819-v

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21:55 min

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April 02, 2012

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, , , , , ,
1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology,Heidelberg University, 2Department of Infectious Diseases, Virology,Heidelberg University

Chapters

  • 00:05Title
  • 03:07Preparation of Plasmid Sets Encoding AAV Capsid Genes
  • 04:20DNase-based Cap Gene Fragmentation
  • 07:03DNA Family Shuffling
  • 11:07Production of Viral Library
  • 13:50Results: Screening and Selection of Individual Chimeric Capsids
  • 20:00Conclusion

Summary

Automatic Translation

Automatic Translation

Vi viser den grundlæggende teknik til molekylær konstruere og udvikle syntetiske Adeno-associerede virus (AAV) genterapivektorer via DNA familie blanding. Desuden leverer vi generelle retningslinjer og repræsentative eksempler på udvælgelse og analyse af individuelle kimære capsider med forbedrede egenskaber på målceller i kultur eller i mus.

Tags

Adeno-associated Viral VectorsAAV Gene TherapyDNA Family ShufflingSynthetic VectorsTherapeutic Human Gene TransferRecombinant VectorsAAV SerotypesNatural VirusesSynthetic AAV VectorsDNA FragmentationAAV Capsid GenesPeptide DisplayHigh-throughput Method

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