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Ingegneria ed evoluzione del sintetico virus adeno-associato (AAV) Vettori Terapia genica attraverso la famiglia Shuffling DNA
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Immunology and Infection
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JoVE Journal Immunology and Infection
Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling
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Ingegneria ed evoluzione del sintetico virus adeno-associato (AAV) Vettori Terapia genica attraverso la famiglia Shuffling DNA

italiano

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28,107 Views

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21:55 min

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April 02, 2012

DOI:

10.3791/3819-v

DOI:
10.3791/3819-v

•

21:55 min
April 02, 2012

•

24 Views
, , , , , ,
1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology,Heidelberg University, 2Department of Infectious Diseases, Virology,Heidelberg University

Summary

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Dimostriamo la tecnica di base per progettare molecolare ed evolvere sintetici virale adeno-associati (AAV) vettori di terapia genica attraverso la famiglia shuffling DNA. Inoltre, fornire linee guida generali ed esempi rappresentativi per la selezione e l'analisi delle singole capsidi chimerici con proprietà migliorate sulle cellule bersaglio in coltura o nei topi.

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Cite this Article

Kienle, E., Senís, E., Börner, K., Niopek, D., Wiedtke, E., Grosse, S., Grimm, D. Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling. J. Vis. Exp. (62), e3819, doi:10.3791/3819 (2012).

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