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Immunology and Infection
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Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

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16,936 Views

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08:52 min

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May 27, 2011

DOI:

10.3791/2736-v

DOI:
10.3791/2736-v

•

08:52 min
May 27, 2011

•

2 Views
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1Center for Cell and Gene Therapy,Baylor College of Medicine

Summary

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A rapid, simple and cost-effective protocol for the generation of donor-derived multivirus-specific CTLs (rCTL) for infusion to allogeneic hematopoietic stem cell transplant (HSCT) recipients at risk of developing CMV, Adv or EBV infections. This manufacturing process is GMP-compliant and should ensure the broader implementation of T-cell immunotherapy beyond specialized centers.

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Gerdemann, U., Vera, J. F., Rooney, C. M., Leen, A. M. Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant. J. Vis. Exp. (51), e2736, doi:10.3791/2736 (2011).

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