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A polido e reforçado Janela afinado crânio para longo prazo de imagens do cérebro de camundongo
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Neuroscience
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JoVE Journal Neuroscience
A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

A polido e reforçado Janela afinado crânio para longo prazo de imagens do cérebro de camundongo

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26,433 Views

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09:49 min

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March 07, 2012

DOI:

10.3791/3742-v

DOI:
10.3791/3742-v

•

09:49 min
March 07, 2012

•

6 Views
, , , ,
1Department of Physics,University of California, San Diego, 2Department of Engineering Science and Mechanics,Pennsylvania State University , 3Department of Neurosurgery,Pennsylvania State University , 4Section of Neurobiology,University of California, San Diego

Summary

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Nós apresentar um método para formar uma janela de imagem no crânio do rato que se estende por milímetro e é estável durante meses sem inflamação do cérebro. Este método é bem adequado para estudos longitudinais de fluxo de sangue, a dinâmica celular, e as células / estrutura vascular utilizando dois fotões microscopia.

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Cite this Article

Shih, A. Y., Mateo, C., Drew, P. J., Tsai, P. S., Kleinfeld, D. A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain. J. Vis. Exp. (61), e3742, doi:10.3791/3742 (2012).

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