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Un lucido e rinforzato Assottigliato cranio-Window a lungo termine Imaging del cervello di topo
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Neuroscience
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JoVE Journal Neuroscience
A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain
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Un lucido e rinforzato Assottigliato cranio-Window a lungo termine Imaging del cervello di topo

italiano

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26,433 Views

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09:49 min

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March 07, 2012

DOI:

10.3791/3742-v

DOI:
10.3791/3742-v

•

09:49 min
March 07, 2012

•

6 Views
, , , ,
1Department of Physics,University of California, San Diego, 2Department of Engineering Science and Mechanics,Pennsylvania State University , 3Department of Neurosurgery,Pennsylvania State University , 4Section of Neurobiology,University of California, San Diego

Summary

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Presentiamo un metodo per formare una finestra di imaging nel cranio topo che si estende millimetri ed è stabile per mesi senza infiammazione cerebrale. Questo metodo è adatto per studi longitudinali di flusso sanguigno, dinamica cellulare, e cellule / struttura vascolare con microscopia a due fotoni.

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Cite this Article

Shih, A. Y., Mateo, C., Drew, P. J., Tsai, P. S., Kleinfeld, D. A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain. J. Vis. Exp. (61), e3742, doi:10.3791/3742 (2012).

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