Journal
/
Neurociência
/
A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

26,433 Views

•

09:49 min

•

March 07, 2012

DOI:

10.3791/3742-v

DOI:
10.3791/3742-v

•

09:49 min
March 07, 2012

•

6 Views
, , , ,
1Department of Physics,University of California, San Diego, 2Department of Engineering Science and Mechanics,Pennsylvania State University , 3Department of Neurosurgery,Pennsylvania State University , 4Section of Neurobiology,University of California, San Diego

Summary

Automatically generated

We present a method to form an imaging window in the mouse skull that spans millimeters and is stable for months without inflammation of the brain. This method is well suited for longitudinal studies of blood flow, cellular dynamics, and cell/vascular structure using two-photon microscopy.

Explore More Videos

Polished And Reinforced Thinned-skull WindowLong-term ImagingMouse BrainIn Vivo ImagingCortical FunctionOptical AccessIntracranial EnvironmentPoRTS WindowOptical ClarityBone Regrowth InhibitionLight Scattering ReductionInflammation ReductionTwo-photon Laser Scanning MicroscopyCerebral Blood FlowCellular FunctionAnesthetized PreparationsAwake PreparationsOptogeneticsBlood Flow Disruption

Read Article

Cite this Article

Shih, A. Y., Mateo, C., Drew, P. J., Tsai, P. S., Kleinfeld, D. A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain. J. Vis. Exp. (61), e3742, doi:10.3791/3742 (2012).

Download .ris file

Copy

Share Video

.

Copy