A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

Andy Y. Shih; Celine Mateo; Patrick J. Drew; Philbert S. Tsai; David Kleinfeld

doi:10.3791/3742

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神経科学

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A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

JoVE Journal
神経科学

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JoVE Journal 神経科学

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

26,433 Views

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09:49 min

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March 07, 2012

DOI:

10.3791/3742-v

DOI:

10.3791/3742-v

•

09:49 min

March 07, 2012

•

6 Views

Andy Y. Shih¹, Celine Mateo¹, Patrick J. Drew^2,3, Philbert S. Tsai¹, David Kleinfeld^1,4

¹物理学科,University of California, San Diego, ²Department of Engineering Science and Mechanics,Pennsylvania State University , ³Department of Neurosurgery,Pennsylvania State University , ⁴Section of Neurobiology,University of California, San Diego

概要

We present a method to form an imaging window in the mouse skull that spans millimeters and is stable for months without inflammation of the brain. This method is well suited for longitudinal studies of blood flow, cellular dynamics, and cell/vascular structure using two-photon microscopy.

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Polished And Reinforced Thinned-skull Window Long-term Imaging Mouse Brain In Vivo Imaging Cortical Function Optical Access Intracranial Environment PoRTS Window Optical Clarity Bone Regrowth Inhibition Light Scattering Reduction Inflammation Reduction Two-photon Laser Scanning Microscopy Cerebral Blood Flow Cellular Function Anesthetized Preparations Awake Preparations Optogenetics Blood Flow Disruption

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A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

概要

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