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A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain
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JoVE Journal 神経科学
A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

DOI:
10.3791/3742-v

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09:49 min

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March 07, 2012

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, , , ,
1物理学科,University of California, San Diego, 2Department of Engineering Science and Mechanics,Pennsylvania State University , 3Department of Neurosurgery,Pennsylvania State University , 4Section of Neurobiology,University of California, San Diego

章

  • 00:05標題
  • 01:40Surgery Preparation and Animal Mounting
  • 04:02The Generation of a Polished and Reinforced Thinned-skull Window
  • 07:14Imaging Preparation
  • 08:20Representative Results for Long-term Imaging and optical Manipulation of the Mouse Cortex
  • 09:30Conclusion

概要

自動翻訳

自動翻訳

We present a method to form an imaging window in the mouse skull that spans millimeters and is stable for months without inflammation of the brain. This method is well suited for longitudinal studies of blood flow, cellular dynamics, and cell/vascular structure using two-photon microscopy.

タグ

Polished And Reinforced Thinned-skull WindowLong-term ImagingMouse BrainIn Vivo ImagingCortical FunctionOptical AccessIntracranial EnvironmentPoRTS WindowOptical ClarityBone Regrowth InhibitionLight Scattering ReductionInflammation ReductionTwo-photon Laser Scanning MicroscopyCerebral Blood FlowCellular FunctionAnesthetized PreparationsAwake PreparationsOptogeneticsBlood Flow Disruption

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