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小鼠腘窝淋巴结的活体成像
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Intravital Imaging of the Mouse Popliteal Lymph Node
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小鼠腘窝淋巴结的活体成像

DOI:
10.3791/3720-v

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08:41 min

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February 08, 2012

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, , ,
1Department of Pediatrics,Case Western Reserve University , 2Department of Pediatrics, Pathology and Biomedical Engineering,Case Western Reserve University

Chapters

  • 00:05Title
  • 01:13Mouse Holder Assembly
  • 01:58Mouse Preparation
  • 03:27Surgery
  • 04:552-Photon Imaging Acquisition
  • 05:55Migration Analysis of GFP+ Lymphocytes in the Popliteal Lymph Node
  • 08:15Conclusion

Summary

Automatic Translation

Automatic Translation

2 - 光子显微镜的最新进展已启用实时<em>在</em>原位成像动物模型的活组织，从而提高我们的能力，探讨在生理和病理条件下细胞行为。在这里，我们勾勒出执行小鼠腘窝淋巴结的活体成像所需的筹备工作。

Tags

Intravital ImagingMousePopliteal Lymph NodeLymph NodesImmune ResponseImmune Cell InteractionsLymphocytesT CellsB CellsNK CellsDendritic CellsMacrophagesBone Marrow-derived Cellular ElementsSelf AntigensForeign AntigensAntigen ReceptorsAdhesion MoleculesChemokinesStromal StructuresFibro-reticular NetworkHigh-resolution Real-time Fluorescent In Vivo ImagingStatic ImagingMorphologyPositionArchitectureLymphocyte TraffickingDynamic Cell BehaviorIntravital Two-photon Laser Scanning

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