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Intravital Imaging of the Mouse Popliteal Lymph Node
Chapitres
- 00:05Titre
- 01:13Mouse Holder Assembly
- 01:58Mouse Preparation
- 03:27Surgery
- 04:552-Photon Imaging Acquisition
- 05:55Migration Analysis of GFP+ Lymphocytes in the Popliteal Lymph Node
- 08:15Conclusion
Recent advances in 2-photon microscopy have enabled real-time de situ imaging of live tissues in animal models, thereby enhancing our ability to investigate cellular behavior in both physiologic and pathologic conditions. Here, we outline the preparations required to perform intravital imaging of the mouse popliteal lymph node.
Tags
Intravital ImagingMousePopliteal Lymph NodeLymph NodesImmune ResponseImmune Cell InteractionsLymphocytesT CellsB CellsNK CellsDendritic CellsMacrophagesBone Marrow-derived Cellular ElementsSelf AntigensForeign AntigensAntigen ReceptorsAdhesion MoleculesChemokinesStromal StructuresFibro-reticular NetworkHigh-resolution Real-time Fluorescent In Vivo ImagingStatic ImagingMorphologyPosteArchitectureLymphocyte TraffickingDynamic Cell BehaviorIntravital Two-photon Laser Scanning
