Recent advances in 2-photon microscopy have enabled real-time de situ imaging of live tissues in animal models, thereby enhancing our ability to investigate cellular behavior in both physiologic and pathologic conditions. Here, we outline the preparations required to perform intravital imaging of the mouse popliteal lymph node.
Liou, H. L. R., Myers, J. T., Barkauskas, D. S., Huang, A. Y. Intravital Imaging of the Mouse Popliteal Lymph Node. J. Vis. Exp. (60), e3720, doi:10.3791/3720 (2012).