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February 08, 2012
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Recent advances in 2-photon microscopy have enabled real-time in situ imaging of live tissues in animal models, thereby enhancing our ability to investigate cellular behavior in both physiologic and pathologic conditions. Here, we outline the preparations required to perform intravital imaging of the mouse popliteal lymph node.
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Intravital ImagingMousePopliteal Lymph NodeLymph NodesImmune ResponseImmune Cell InteractionsLymphocytesT CellsB CellsNK CellsDendritic CellsMacrophagesBone Marrow-derived Cellular ElementsSelf AntigensForeign AntigensAntigen ReceptorsAdhesion MoleculesChemokinesStromal StructuresFibro-reticular NetworkHigh-resolution Real-time Fluorescent In Vivo ImagingStatic ImagingMorphologyPositionArchitectureLymphocyte TraffickingDynamic Cell BehaviorIntravital Two-photon Laser Scanning
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Liou, H. L. R., Myers, J. T., Barkauskas, D. S., Huang, A. Y. Intravital Imaging of the Mouse Popliteal Lymph Node. J. Vis. Exp. (60), e3720, doi:10.3791/3720 (2012).
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