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Ibridazione<em> In situ</em> Delle ghiandole salivari, ovaie, e gli embrioni di Vector zanzare
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
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Ibridazione<em> In situ</em> Delle ghiandole salivari, ovaie, e gli embrioni di Vector zanzare

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16,179 Views

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10:58 min

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June 28, 2012

DOI:

10.3791/3709-v

DOI:
10.3791/3709-v

•

10:58 min
June 28, 2012

•

10 Views
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1Department of Molecular Biology and Biochemistry,University of California, Irvine, 2Department of Microbiology and Molecular Genetics,University of California, Irvine

Summary

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Temporali e spaziali analisi di espressione genica hanno un ruolo cruciale nella genomica funzionale. Whole-mount ibridazione<em> In situ</em> È utile per determinare la localizzazione dei trascritti all'interno dei tessuti e compartimenti subcellulari. Qui delineare una ibridazione<em> In situ</emProtocollo> con modifiche di tessuti specifici in zanzare.

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Hybridization In SituSalivary GlandsOvariesEmbryosVector MosquitoesPathogensTranscriptsGene RegulatorsMosquito-borne Disease TransmissionEgg DevelopmentGene Expression RegulationSpatial InformationAccumulation PatternsFemale Salivary GlandsOvaries And Embryos Subcellular CompartmentsMosquito Reproduction And DevelopmentRNA Probe PreparationFixation And Hybridization

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Cite this Article

Juhn, J., James, A. A. Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes. J. Vis. Exp. (64), e3709, doi:10.3791/3709 (2012).

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