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Immunologie und Infektion
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Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

16,263 Views

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10:58 min

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June 28, 2012

DOI:

10.3791/3709-v

DOI:
10.3791/3709-v

•

10:58 min
June 28, 2012

•

10 Views
,
1Department of Molecular Biology and Biochemistry,University of California, Irvine, 2Department of Microbiology and Molecular Genetics,University of California, Irvine

Summary

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Temporal and spatial gene expression analyses have a crucial role in functional genomics. Whole-mount hybridization in situ is useful for determining the localization of transcripts within tissues and subcellular compartments. Here we outline a hybridization in situ protocol with modifications for specific target tissues in mosquitoes.

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Hybridization In SituSalivary GlandsOvariesEmbryosVector MosquitoesPathogensTranscriptsGene RegulatorsMosquito-borne Disease TransmissionEgg DevelopmentGene Expression RegulationSpatial InformationAccumulation PatternsFemale Salivary GlandsOvaries And Embryos Subcellular CompartmentsMosquito Reproduction And DevelopmentRNA Probe PreparationFixation And Hybridization

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Juhn, J., James, A. A. Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes. J. Vis. Exp. (64), e3709, doi:10.3791/3709 (2012).

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