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Immunology and Infection
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Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

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16,263 Views

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10:58 min

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June 28, 2012

DOI:

10.3791/3709-v

DOI:
10.3791/3709-v

•

10:58 min
June 28, 2012

•

10 Views
,
1Department of Molecular Biology and Biochemistry,University of California, Irvine, 2Department of Microbiology and Molecular Genetics,University of California, Irvine

Summary

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Temporal and spatial gene expression analyses have a crucial role in functional genomics. Whole-mount hybridization in situ is useful for determining the localization of transcripts within tissues and subcellular compartments. Here we outline a hybridization in situ protocol with modifications for specific target tissues in mosquitoes.

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Hybridization In SituSalivary GlandsOvariesEmbryosVector MosquitoesPathogensTranscriptsGene RegulatorsMosquito-borne Disease TransmissionEgg DevelopmentGene Expression RegulationSpatial InformationAccumulation PatternsFemale Salivary GlandsOvaries And Embryos Subcellular CompartmentsMosquito Reproduction And DevelopmentRNA Probe PreparationFixation And Hybridization

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Juhn, J., James, A. A. Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes. J. Vis. Exp. (64), e3709, doi:10.3791/3709 (2012).

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