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Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
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Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
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10:58 min
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June 28, 2012
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Özet
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Temporal and spatial gene expression analyses have a crucial role in functional genomics. Whole-mount hybridization in situ is useful for determining the localization of transcripts within tissues and subcellular compartments. Here we outline a hybridization in situ protocol with modifications for specific target tissues in mosquitoes.
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Hybridization In SituSalivary GlandsOvariesEmbryosVector MosquitoesPathogensTranscriptsGene RegulatorsMosquito-borne Disease TransmissionEgg DevelopmentGene Expression RegulationSpatial InformationAccumulation PatternsFemale Salivary GlandsOvaries And Embryos Subcellular CompartmentsMosquito Reproduction And DevelopmentRNA Probe PreparationFixation And Hybridization
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Juhn, J., James, A. A. Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes. J. Vis. Exp. (64), e3709, doi:10.3791/3709 (2012).
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