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Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
JoVE Journal
免疫学と感染
This content is Free Access.
JoVE Journal 免疫学と感染
Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

DOI:
10.3791/3709-v

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10:58 min

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June 28, 2012

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,
1Department of Molecular Biology and Biochemistry,University of California, Irvine, 2Department of Microbiology and Molecular Genetics,University of California, Irvine

章

  • 00:05標題
  • 01:25In situ Hybridization
  • 03:27Glycerol Mounting
  • 05:30Dechorionation, Fixation, and Endochorion Disruption
  • 08:04Peeling
  • 09:44Representative In situ 結果
  • 10:36Conclusion

概要

自動翻訳

自動翻訳

Temporal and spatial gene expression analyses have a crucial role in functional genomics. Whole-mount hybridization in situ is useful for determining the localization of transcripts within tissues and subcellular compartments. Here we outline a hybridization in situ protocol with modifications for specific target tissues in mosquitoes.

タグ

Hybridization In SituSalivary GlandsOvariesEmbryosVector MosquitoesPathogensTranscriptsGene RegulatorsMosquito-borne Disease TransmissionEgg DevelopmentGene Expression RegulationSpatial InformationAccumulation PatternsFemale Salivary GlandsOvaries And Embryos Subcellular CompartmentsMosquito Reproduction And DevelopmentRNA Probe PreparationFixation And Hybridization

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