JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Neuroscience
/
人类胶质母细胞瘤肿瘤细胞的分离和扩大使用的神经球含量
JoVE Journal
Neuroscience
Author Produced
This content is Free Access.
JoVE Journal
Neuroscience
Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay
Please note that all translations are automatically generated.
Click here for the English version.
人类胶质母细胞瘤肿瘤细胞的分离和扩大使用的神经球含量
DOI:
10.3791/3633-v
•
09:24 min
•
October 30, 2011
•
Hassan Azari
2
,
Sebastien Millette
,
Saeed Ansari
,
Maryam Rahman
,
Loic P. Deleyrolle
,
Brent A. Reynolds
1
Department of Neurosurgery
,
University of Florida
,
2
Department of Anatomical Sciences
,
Shiraz University of Medical Sciences
Chapters
00:05
Title
00:26
Introduction
00:49
Primary GBM Neurosphere Culture
05:06
Passaging GBM-Derived Neurospheres
07:43
Representative Results
08:41
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
这个视频协议演示的隔离和扩大干细胞的神经球的实验文化方法如手术切除人脑胶质瘤mutliforme(GBM)的肿瘤组织使用。
Tags
Isolation
Expansion
Human Glioblastoma Multiforme
Tumor Cells
Neurosphere Assay
Stem-like Cells
Breast
Lung
Colon
Prostate
Brain
Tumor Initiating Cell Population(s)
Tumor Formation
Progression
Recurrence
Therapeutic Approaches
Neurosphere Culture Method
Chemical Digestion
Mechanical Dissociation
Single Cell Suspension
NSA Culture
Primary Neurospheres
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
成年鼠神经干细胞的分离和扩大使用神经干含量
建立小鼠胚胎神经干细胞培养神经球含量
人力蕈状味觉乳头细胞的分离和培养
谱系重编程的成人大脑的周细胞源性细胞诱导成神经细胞
使用F9稀土LACZ基于Cell的报告基因检测在E8.5胚胎和神经球文化相对维甲酸水平的定量测量
使用微注射在人赫拉细胞中分体血球定位分析
产后神经球的分离和扩张 (P1+3) 小鼠神经原性球
人类诱导多能干细胞脑微血管内皮细胞的衍生、扩张、冷冻保存和特征化
穿梭盒测定作为使用成人斑马鱼进行学习和记忆研究认知评估的关联学习工具
从人包皮中分离、培养和表征原代施旺细胞、角质形成细胞和成纤维细胞
Read Article