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Neurosphere Testi İnsan Glioblastoma Multiforme Tümör Hücreleri İzolasyon ve Genişleme
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09:24 min
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October 30, 2011
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Bu video protokolü neurosphere tahlil kültürü yöntemi kullanılarak insan glioblastoma mutliforme cerrahi rezeksiyon (GBM) tümör dokusu hücreleri gibi izolasyon ve kök genişlemesi göstermektedir.
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IsolationExpansionHuman Glioblastoma MultiformeTumor CellsNeurosphere AssayStem-like CellsBreastLungColonProstateBrainTumor Initiating Cell Population(s)Tumor FormationProgressionRecurrenceTherapeutic ApproachesNeurosphere Culture MethodChemical DigestionMechanical DissociationSingle Cell SuspensionNSA CulturePrimary Neurospheres
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Cite this Article
Azari, H., Millette, S., Ansari, S., Rahman, M., Deleyrolle, L. P., Reynolds, B. A. Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay. J. Vis. Exp. (56), e3633, doi:10.3791/3633 (2011).
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