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Immunology and Infection
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組換えアデノ随伴ウイルスベクターの産生と力価測定
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Production and Titering of Recombinant Adeno-associated Viral Vectors
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組換えアデノ随伴ウイルスベクターの産生と力価測定

日本語

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60,958 Views

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08:35 min

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November 27, 2011

DOI:

10.3791/3348-v

DOI:
10.3791/3348-v

•

08:35 min
November 27, 2011

•

23 Views
, , , ,
1School of Medical Sciences, College of Life Sciences and Medicine,University of Aberdeen, 2Translational Neuroscience Facility and Department of Physiology,School of Medical Sciences, University of New South Wales, 3Department of Biochemistry and Molecular Biophysics,Columbia University

Summary

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組換えアデノ随伴ウイルス（rAAVs）ベクトルはのためにますます貴重になってきています<em> in vivoで</em>動物での試験。我々はrAAVsが実験室で製造することができるとどのようにこれらのベクトルが生成される感染性粒子の数の正確な読みを与えるために滴定することができる方法について説明します。

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McClure, C., Cole, K. L. H., Wulff, P., Klugmann, M., Murray, A. J. Production and Titering of Recombinant Adeno-associated Viral Vectors. J. Vis. Exp. (57), e3348, doi:10.3791/3348 (2011).

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