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Immunology and Infection

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JoVE Journal Immunology and Infection
Production and Titering of Recombinant Adeno-associated Viral Vectors
 

Production and Titering of Recombinant Adeno-associated Viral Vectors

Article DOI: 10.3791/3348-v • 08:35 min • November 27th, 2011
November 27th, 2011 •
*1, *1, 1, 2, 1,3
1School of Medical Sciences, College of Life Sciences and Medicine, University of Aberdeen, 2Translational Neuroscience Facility and Department of Physiology, School of Medical Sciences, University of New South Wales, 3Department of Biochemistry and Molecular Biophysics, Columbia University
* These authors contributed equally

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Recombinant adeno-associated virus (rAAVs) vectors are becoming increasingly valuable for in vivo studies in animals. We describe how rAAVs can be produced in the laboratory and how these vectors can be titered to give an accurate reading of the number of infectious particles produced.

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Recombinant Adeno-associated Viral Vectors AAV In Vivo Studies Human Clinical Trials Transduction Profile Immune Response Transgene Expression Gene Delivery RAAVs Safety Profile Chimeric RAAVs AAV1 AAV2 Affinity Chromatography Infectious Properties Heparin Column Purification Viral Titer
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