Rivista
/
Immunologia e infezioni
/
Production and Titering of Recombinant Adeno-associated Viral Vectors
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Production and Titering of Recombinant Adeno-associated Viral Vectors

Production and Titering of Recombinant Adeno-associated Viral Vectors

60,960 Views

•

08:35 min

•

November 27, 2011

DOI:

10.3791/3348-v

DOI:
10.3791/3348-v

•

08:35 min
November 27, 2011

•

23 Views
, , , ,
1School of Medical Sciences, College of Life Sciences and Medicine,University of Aberdeen, 2Translational Neuroscience Facility and Department of Physiology,School of Medical Sciences, University of New South Wales, 3Department of Biochemistry and Molecular Biophysics,Columbia University

Summary

Automatically generated

Recombinant adeno-associated virus (rAAVs) vectors are becoming increasingly valuable for in vivo studies in animals. We describe how rAAVs can be produced in the laboratory and how these vectors can be titered to give an accurate reading of the number of infectious particles produced.

Explore More Videos

Recombinant Adeno-associated Viral VectorsAAVIn Vivo StudiesHuman Clinical TrialsTransduction ProfileImmune ResponseTransgene ExpressionGene DeliveryRAAVsSafety ProfileChimeric RAAVsAAV1AAV2Affinity ChromatographyInfectious PropertiesHeparin Column PurificationViral Titer

Read Article

Cite this Article

McClure, C., Cole, K. L. H., Wulff, P., Klugmann, M., Murray, A. J. Production and Titering of Recombinant Adeno-associated Viral Vectors. J. Vis. Exp. (57), e3348, doi:10.3791/3348 (2011).

Download .ris file

Copy

Share Video

.

Copy