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Produzione e titolazione di vettori virali ricombinanti adeno-associato
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Production and Titering of Recombinant Adeno-associated Viral Vectors
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Produzione e titolazione di vettori virali ricombinanti adeno-associato

italiano

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60,960 Views

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08:35 min

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November 27, 2011

DOI:

10.3791/3348-v

DOI:
10.3791/3348-v

•

08:35 min
November 27, 2011

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23 Views
, , , ,
1School of Medical Sciences, College of Life Sciences and Medicine,University of Aberdeen, 2Translational Neuroscience Facility and Department of Physiology,School of Medical Sciences, University of New South Wales, 3Department of Biochemistry and Molecular Biophysics,Columbia University

Summary

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Ricombinante adeno-associato virus (rAAVs) vettori stanno diventando sempre più prezioso per<em> In vivo</em> Gli studi su animali. Ci descrivono come rAAVs possono essere prodotti in laboratorio e come questi vettori possono essere titolati a dare una lettura precisa del numero di particelle infettive prodotte.

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McClure, C., Cole, K. L. H., Wulff, P., Klugmann, M., Murray, A. J. Production and Titering of Recombinant Adeno-associated Viral Vectors. J. Vis. Exp. (57), e3348, doi:10.3791/3348 (2011).

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